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KMID : 0371619940100010051
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Journal of Wonkwang Medical Science
1994 Volume.10 No. 1 p.51 ~ p.66
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A Study on the Cytotoxicity of Lidocaine, Tetracaine and Bupivacaine in the Cultured Rat Myocardial Cells
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Chang Jeong-Soo
Choi Min-Kyu
Oh Jae-Min
Kim Jong-Young
Chung Yeun-Tai
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Abstract
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In an attempt to evaluate the cytotoxicity for lidocaine, tetracaine and bupivacaine, beating rates, tetrazolium MTT and lactate dehydrogenase activity were investigated in the medium containing the local anesthetic drugs for 24 hours after the neonatal rat myocardial cells were cultured for 72 hours. Light and electron microscopic studies were also carried out. The results were as follows: 1. Beating rates decreased dose-dependently. and beating myocardial cells were not observed at 10^-3M concentration of tetracaine and bupivacaine, and also beating activities were not recovered after 24hrs. 2. MTT_50 values were 995 μM in tetracaine and 983 μM in bupivacaine. 3. The amount of lactate dehydrogenase released into the medium at 10^-3M concentration were 123% in lidocaine, 156% in tetracaine and 182% in bupivacaine compared with control cells. 4. In the light microscopy, myocardial cells were decreased in number dosede-pendently, and showed a few cytoplasmic processes at 10^-3 M concentration of tetracaine and bupivacaine. 5. In electron microscopy, myocardial cells treated with tetracaine and bupivacaine showed destructed mitochondria and many dense bodies. These results suggest that high concentration of tetracaine and bupivacaine (10^-3M) induce remarkable toxicity compared with lidocaine on the cultured rat myocardial cells.
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